Measurement of Unconjugated 17β-Estradiol in Plasma by Competitive Protein Binding

Functional tests of adrenal axis in children with measurement of plasma cortisol by competitive protein binding.

Barnes, N. D., Joseph, J. M., Atherden, S. M., and Clayton, B. E. (1972). Archives of Disease in Childhood, 47, 66. Functional tests of adrenal axis in children with measurement of plasma cortisol by competitive protein binding. The competitive protein binding assay is the method of choice for estimation of plasma cortisol in children. Resting plasma cortisol levels in children without evidence...

Improved measurement of corticosteroids in plasma and urine by competitive protein-binding radioassay.

In the assay of corticosteroids in plasma and urine by competitive protein binding, the use of horse serum or plasma as a source of assay protein gives better sensitivity and somewhat better specificity for cortisol than do previously described procedures. Five to 10 zl of plasma unknown or 50 to 100 tI of urine unknown can be used. Precision for the standard curve is 0.12 ng in the range 0-2.0...

Quantitation of plasma testosterone by improved competitive protein-binding technique.

A method is described for quantitation of testosterone in plasma from females and males. The principal operations of extraction, chromatographic fractionation, and competitive protein-binding assay can be completed for eight duplicate samples in a single day. Experimental data used in the development of the test and the rationale for the specific operations and conditions are presented. The spe...

Immunomodulation by 17β-Estradiol in bivalve hemocytes

In mammals, estrogens have both doseand cell-type specific effects on immune cells and may act as pro-inflammatory and anti-inflammatory stimuli depending on the setting. Evidence has been provided that in the bivalve mollusc Mytilus the natural estrogen 17β-estradiol (E2) can affect neuro-immune functions. In this work the immunomodulatory role of E2 in Mytilus hemocytes, the cells responsible...

Plasma Fluctuation in Estradiol-17β and Bone Resorption Markers around